One hundred c.c. of the wine are taken, evaporated to about 50 c.c. to remove the alcohol, and transferred to a separator. The liquid is first made slightly acid with dilute sulphuric acid and extracted twice with ether, the ethereal solutions being separated, mixed, washed twice with a little water, and the washings added to the main quantity. This is now made alkaline with ammonia and extracted three times with chloroform, using about 20 c.c. of solvent each time. The chloroformic solutions are passed through a small filter into a tared beaker, the filter washed with a little chloroform, and the solution evaporated down to a bulk of about 2 c.c. To this are added 5 c.c. of ether, and the evaporation is continued to dryness at a temperature not exceeding 80°, the residual alkaloids being then weighed, after cooling in a desiccator.