In May 1910, five experiments were made with a vegetable protein substance called "glidine,"2 which is claimed to be the gliadin of wheat. As will be seen from table 50 (page 124), this food material had a protein content of approximately 87 per cent. An unfortunate feature of the experiments with glidine was the fact that the subjects found it almost impossible to eat any considerable amount. The largest amount taken was 70 grams, which was used in two experiments; in three experiments the subject took only 45 grams. All the observations were made with the chair calorimeter. The statistical data not included in the tables or the discussion of the experiments are given here:

L. E. E., 8h40m A. M. To 3h49m P. M., May 3, 1910. 59.6 Kilograms. 2 Basal Periods

Basal periods ended 10h40m a. m.; food periods began 11h49m a. m. Subject unable to take a large amount of glidine without nausea. Urinated 6h30m, 8h47m, 10h45m a. m., 12h49m, 2h49m, 3h49m p. m. Drank 85 grams water 12h49m p. m. Asleep 12h04m p. m. to 12h42m p. m. and lh32m p. m. to 2h08m p. m.; restless at other times. Rectal thermometer slipped out of position in third food period; temperature records not available after 12h49m p. m. Basal periods: body-temperature, 36.90°, 36.81°, 36.93° C; pulse rate, 56; respiration rate, 16. Food periods: body-temperature, 36.92°, 36.94° C; pulse rate, 55; respiration rate, 17.

L. E. E., 8h31m A. M. To 2h43m P. M., May 11, 1910. 59.2 Kilograms. 2 Basal Periods

Fasting periods ended at 10h31m a. m.; food periods began at 11h43m a. m. Subject defecated and urinated at 6h45m a. m.; urinated at 10h31m a. m. and 2h45m p. m. In first part of first basal period, there was a decided movement and subject was cautioned to keep quiet. Asleep at end of period; awakened at beginning of next period. Rectal thermometer not used after lh47m p. m. Basal periods: pulse rate, 56; respiration rate, 17. Food periods: pulse rate, 57; respiration rate, 18.

J. J. C, 9h31m A. M. To 4h43m P. M., May 9, 1910. 64.5 Kilograms. 2 Basal Periods

Basal periods ended llh31m a. m.; food periods began 12h43m p. m. Subject urinated 6h45m, 11h36m a. m., 3h53m, 4h55m p. m. Asleep 11h40m a. m., lh28m p. m., lh48m p. m. and was awakened. Basal periods: body-temperature, 36.6°, 36.69°, 36.7° C.; pulse rate, 60; respiration rate, 19. Food periods: body-temperature, 36.79°, 36.78°, 36.80°, 36.77°, 36.85° C; pulse rate, 59; respiration rate, 19.

J. R., 8h38m A. M. To 3h2m P. M., May 5, 1910. 70.1 Kilograms. 2 Basal Periods

Basal periods ended 10h38m a. m.; food periods began 11h22m a. m. Urinated 7 a. m., 10h40m a. m., 3h30m p. m. Very sleepy during greater part of afternoon and asleep just before 2h22m p. m. but awoke shortly afterwards. Basal periods: body-temperature, 37.01°, 37.01°, 37.09° C; pulse rate, 65; respiration rate, 15. Food periods: body-temperature, 37.22°, 37.14°, 37.28°, 37.19°, 37.37° C.; pulse rate, 68; respiration rate, 15.

1It is of interest to note here that Aub and DuBois, in a recent research, found larger increases in the metabolism following the ingestion of beefsteak with abnormal individuals (a dwarf and a legless man) than with normal individuals of greater weight and body-surface area. (Aub and DuBois, Arch. Intern. Med., 1917, 19, p. 840).

2Street, Ann. Rept. Conn. Agr. Exp. Sta., 1913.

J. R., 8h37m A. M. To 3h42m P. M., May 10, 1910. 71.0 Kilograms. 2 Basal Periods

Basal periods ended 10h37m a. m.; food periods began llh42m a. m. Subject felt chilly at first. Urinated 7 a. m., 10h37m a. m. and 2h46m p. m. Basal periods: body-temperature, 37.00°, 36.90°, 37.08° C; pulse rate, 66; respiration rate, 16. Food periods: body-temperature, 37.23°, 37.56°, 37.47°, 37.38°, 37.46° C; pulse rate, 75; respiration rate, 16.

Discussion Of Experiments

L. E. E., May 3, 1910

The subject took 45 grams of glidine suspended in 110 grams of water, with a nitrogen content of 6.24 grams; the results obtained are given in table 219. The basal value was found on the same day, immediately prior to the ingestion of the glidine. A marked increment in carbon-dioxide production and oxygen consumption was noted in all the periods of the experiment and an increase in heat production in the first two periods; in the last two periods the values for the heat production were within 1 calorie of the basal value.

Table 219. - L. E. E., May 3, 1910. Sitting. (1-Hour Periods). Glidine1

Amount, 45 grams; nitrogen, 6.24 grams; total energy, 223 cals.

Fuel value: Total, 168 cals.; from protein, 95 p. ct.; from fat, 2 p. ct.; from carbohydrates, 3 p. ct. Basal values (May 3, 1910): CO2, 25 grams; O2, 21.5 grams; heat2, 78 cals.; respiratory quotient, 0.84. Nitrogen in urine, 0.51 gram per hour.

1Subject took glidine in 110 grams of water.

2Heat eliminated corrected for change in body-weight, but not for change in body-temperature.