A. H. M., March 23, 1906

Following the ingestion of 599 grams milk and 8 grams of lime-water, with a fuel value of 385 calories, an increment was obtained in the carbon-dioxide production in the first three periods, with a value below basal in the fourth period. (See table 233.) The oxygen consumption was somewhat irregular and also showed values below basal in the fourth period, with a total increment of 11 grams for the 8 hours. The heat production in the first three periods increased measurably, but here again a value below the base-fine was obtained in the last period. It is significant that in the fourth period values below basal are observed for all of the three factors of metabolism, strongly implying that the basal value determined on February 12 and 14, 1906, was erroneous and that a true basal value for this day would have been nearer to that found in this period of the experiment. In that case the increment due to the ingestion of milk would have been greater than here recorded. It is clear that even with this imperfect base-line there was a very measurable increment due to the ingestion of milk, especially in the first three periods. Since the total nitrogen intake was but 3.17 grams, it is probable that this effect upon the metabolism should not be ascribed solely to the protein in the milk. In our study of the effect of carbohydrate ingestion, lactose, which is present in milk in considerable amounts, was noted as having a positive effect upon the metabolism; hence we probably have here a summation effect of the protein and lactose. While the three experiments as a whole are not especially satisfactory as duplicate experiments, they are uniform in indicating a positive increment due to the ingestion of milk.

Table 233. - A. H. M., March 23, 1906. Sitting. (2-Hour Periods). Milk (Whole)

Amount, 599 grams;1 nitrogen, 3.17 grams; total energy, 412 cals.

Fuel value: Total, 385 cals.; from protein, 19 p. ct.; from fat, 52 p. ct.; from carbohydrates, 29 p. ct.

Nitrogen in urine, 1.25 grams per 2 hours.2 Basal values (February 12 and 14, 1906): CO2, 45 grams; O2, 40 grams; heat, 142 cals.

1Also 8 grams lime-water.

2Sample included amount for about 2 hours without food preceding experiment.

Respiration Experiment

Only one respiration experiment was made with milk, this being a part of the later investigations in Boston. The universal respiration apparatus was employed, with experimental periods of the usual 15-minute length. The basal value was determined in several periods immediately preceding the taking of the food.

H. F. T., July 14, 1911

The amount of milk taken by this subject was 500 grams, with a fuel value of 358 calories. This energy content was somewhat less than that of the milk used in the calorimeter experiments, although derived from the three nutrients in the same proportions as in those experiments. The details of the experiment are given in table 234. The maximum increase in metabolism, which occurred inside of the first hour, was relatively slight, the heat production rising from 0.91 to 1.01 calories per minute. The basal value was reached in 3 hours. Although unaccompanied by respiration experiments with other subjects or with the same subject, the results of this experiment are of interest for comparison with the data obtained in the calorimeter experiments with this common food material.

Table 234. - H. F. T., July 14, 1911. Lying. (Values Per Minute). Milk (Whole)

Amount, 500 grams; nitrogen, 2.64 grams; total energy, 381 cals.

Fuel value, 358 cals.; from protein, 19 p. ct.; from fat, 52 p. ct.; from carbohydrates, 29 p. ct.

1Subject had eaten 206 grams beefsteak at 12 midnight, July 13. 2Subject drank milk between 10h07m and 10h40m a. m.